Figure 2.

Altering OSM-11 DOS co-ligand levels affect the paralysis rate on aldicarb. (A) Response of osm-11(rt142null) animals to aldicarb. Animals that lack the DOS co-ligand OSM-11 are hypersensitive to aldicarb, similar to lin-12(null) animals. (B) Overexpression of OSM-11 in adults by heat-shock causes resistance to aldicarb compared to heat-shocked wild type controls. (C) osm-11 knockdown by feeding RNAi recapitulates aldicarb hypersensitivity seen in osm-11(rt142null) animals. Knockdown of osm-11 starting from late L2 is enough to cause increased hypersensitivity to aldicarb in adults and restoring osm-11 expression after late L2 stage reverses this hypersensitivity. (hs: heat-shock, no hs: mock heat-shock, at least three independent trials for each experiment, n > 30 for all conditions, error bars represent SEM *: log-rank p-value < 0.05, **: log-rank p-value < 0.01, ***: log-rank p-value < 0.001, ns: no significant difference.)