Table 1. Aldicarb response of ligand or co-ligand mutant animals. Loss of OSM-7, OSM-11 or DSL-3 phenocopies LIN-12 loss, whereas decreased LAG-2, DOS-1 or DSL-6 results in resistance to aldicarb similarly to decreased glp-1 function. n/a indicates “not available”. At least 10 animals tested for dos-2, arg-1, dsl-2, dsl-4 and dsl-5. For RNAi experiments, three independent trials were conducted using the neuronal RNAi-sensitive strain eri-1(mg366); lin-15B(n744), with a total of at least 30 animals for each knocked-down gene. For non-wild type responses, aldicarb-induced paralysis rates are in Figure S2. For wild type responses the raw data are available in supplements.

Gene	Function	Allele	Effect on function	Aldicarb Response
osm-7	DOS co-ligand	tm2256	null	hypersensitive
osm-11	DOS co-ligand	rt142	null	hypersensitive
dos-1	DOS co-ligand	ok2398	null	resistant
dos-2	DOS co-ligand	tm4515	putative null	wild type
dos-3	DOS co-ligand	n/a	RNAi knockdown	wild type
lag-2	DSL ligand	q420	loss of function	resistant
arg-1	DSL ligand	ok3127	putative null	wild type
apx-1	DSL ligand	n/a	RNAi knockdown	wild type
dsl-1	DSL ligand	ok810	null	wild type
dsl-2	DSL ligand	tm1805	putative null	wild type
dsl-3	DSL ligand	ok3411	putative null	hypersensitive
dsl-4	DSL ligand	ok1020	putative null	wild type
dsl-5	DSL ligand	ok588	putative null	wild type
dsl-6	DSL ligand	ok2265	putative null	resistant
dsl-7	DSL ligand	n/a	RNAi knockdown	wild type