Figure 2.

Reduction of bacterial load on different types of hard surfaces treated with specific phages. One hundred CFU of each ATCC bacterial strain (S. aureus, E. coli, and P. aeruginosa) were spread on the indicated sterile surfaces, allowed to dry, and subsequently treated with the specific phages, suspended in PBS, at the indicated MOI. After 1, 3, 6, and 24 hours at room temperature, residual viable cells were measured by Rodac sampling with specific selective media and subsequent CFU count after 24 hours of incubation at 37°C. Negative CTR(−) samples were treated with PBS alone. Positive CTR(+) samples were treated with denatured alcohol. Results represent the mean ± SD of triplicate samples in two independent assays per bacterial target.

Abbreviations: ATCC, American Type Culture Collection; CFU, colony forming units; PBS, phosphate-buffered saline; MOI, multiplicity of infection; CTR, control.