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. 2018 Mar 14;20(9):1173–1184. doi: 10.1093/neuonc/noy037

Fig. 2 .

Fig. 2

OIP5 is required for proliferation of glioblastoma cells and regulates cell cycle progression. (A) Western blot analysis of OIP5 expression in LN-229, U-87 MG, and A172 cell lines expressing shGFP, shOIP5#1, or shOIP5#2. (B) Growth curves of LN-229, U-87 MG, and A172 cells expressing shGFP, shOIP5#1, or shOIP5#2. (C) Representative fluorescent micrographs and quantification for BrdU staining of LN-229, U-87 MG, and A172 cells expressing shGFP, shOIP5#1, or shOIP5#2. (D) Quantification of cell population in each phase of LN-229, U-87 MG, and A172 cells expressing shGFP, shOIP5#1, or shOIP5#2. (E) Protein expression of the indicated genes in LN-229, U-87 MG, and A172 cells expressing shGFP, shOIP5#1, or shOIP5#2. Data were analyzed using 2-tailed Student’s t-tests, *P < 0.05, **P < 0.01. Scale bars 20 μm.