Fig. 4 .

OIP5 is required for glioblastoma colony formation in vitro and tumor formation in vivo. (A) Colony formation ability of LN-229 and U-87 MG cells expressing shGFP, shOIP5#1, or shOIP5#2. (B) Colony formation ability of the OIP5-rescued OIP5 knockdown cells. (C) Orthotopic implantation was performed after OIP5 knockdown in LN-229 and U-87 MG cells. Representative images of the hematoxylin and eosin staining are presented. (D) Survival rates of mice were analyzed after orthotopic implantation. Data were analyzed using 2-tailed Student’s t-tests, **P < 0.01. Scale bars Panels A and B: 50 μm; Panel C: 3 mm.