. Author manuscript; available in PMC: 2018 Aug 2.

Published in final edited form as: J Water Resour Prot. 2017 Oct 31;9(11):1–29. doi: 10.4236/jwarp.2017.911083

Table 1.

Oligonucleotide PCR primer sequences for clone sequencing.

Target	Oligo name	Sequences (5′–3′)	Tm (°C) bp		Reference
mcyE or ndaF, for total MC producers	HEPf	TTTGGGGTTAACTTTTTTGGGCATAGTC	56	470	Jungblut and Neilan, 2006
mcyE or ndaF, for total MC producers	HEPr	AATTCTTGAGGCTGTAAATCGGGTTT	56	470	Jungblut and Neilan, 2006
mcyA for MC producers of Microcystis,	CD1f	AAAAGTGTTTTATTAGCGGCTCAT	56	302	Hisbergues, 2003
Planktothrix and Dolichospermum	CD1r	ATCCAGCAGTTGAGCAAGC	56	302	Hisbergues, 2003
cyrA, Cylindrospermopsin	cyrAf	GTCTGCCCACGTGATGTTATGAT	60	71	Al-Tebrineh et al., 2012
cyrA, Cylindrospermopsin	cyrAr	CGTGACCGCCGTGACA	60	71	Al-Tebrineh et al., 2012
cyrL	cyrLf	CAGGCTTATCTGCAACAACATTTCT	56	813	Jiang et al., 2014
cyrL	cyrLr	CGGTTTATCAGTTCCAGAGTATCCA	56	813	Jiang et al., 2014
rpo, C. raciborskii	Cy12	GGCATTCCTAGTTATATTGCCATACTA	56	234	Wilson et al., 2000
rpo, C. raciborskii	Cy14	GCCCGTTTTTGTCCCTTTGCTGC	56	234	Wilson et al., 2000
anaC	anaC-gen F	TCTGGTATTCAGTCCCCTCTAT	58	366	Rantala-Ylinen et al., 2011
anaC	anaC-gen R	CCCAATAGCCTGTCATCAA	58	366	Rantala-Ylinen et al., 2011
sxtA, Dolichospermum,	sxtAf	GGAGTGGATTTCAACACCAGAA	60	148	Al-Tebrineh et al., 2012
Cylindrospermopsin, Lyngbya	sxtAr	GTTTCCCAGACTCGTTTCAGG	60	148	Al-Tebrineh et al., 2012
Nda, toxicNostoc	ndaF	GTG ATT GAA TTT CTT GGT CG	61	188	Koskenniemi, 2007
Nda, toxicNostoc	ndaR	GGA AAT TTC TAT GTC TGA CTC AG	61	188	Koskenniemi, 2007
Nts, total Nostoc	NTSf	TGTGATGCAAATCTCA(C}A)A	56	200	Moffitt, 2001; Neilan et al., 1997
Nts, total Nostoc	1492R	TACGGCTACCTTGTTACGAC	56	200	Moffitt, 2001; Neilan et al., 1997