Table 2.
Conjugation between the 5’-activated supported trinucleotide 2 and several biomolecules. Reagents and conditions: (a) (i) biomolecule (2.0 equiv), BMT (2.0 equiv), CH2Cl2/CH3CN/DMF (20:2:1, v/v/v), rt, 30 min; (ii) 0.67% BPO/DMP/CH2Cl2, rt, 30 min. (b) (i) 5% DCA/CH2Cl2, rt, 5 min; (ii) NH3 aq/EtOH (3:1, v/v), 70 °C, 3 h; (iii) TEA 3HF/DMF, rt, 24 h.
 | ||||
| entry | biomolecule | conjugation yield (%)a | bioconjugate | deprotection yield (%)b |
| 1 |
 6 |
n.d. |
 8 |
– |
| 2 |
 7 |
70 |
 8 |
67 |
| 3c |
 9 |
68 |
 11 |
28 |
| 4c |
 10 |
96 |
 12 |
41 |
aYields were determined by 31P NMR and HPLC analyses. bYields were determined by HPLC analysis. cCH2Cl2/CH3CN (10:1. v/v) was used as a solvent.