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. 2018 Apr 5;126(4):047002. doi: 10.1289/EHP1575

Figure 3.

Figure 3A represents the dosing regimen timeline and two bar graphs showing body weight in grams and uterine to body weight ratio on the 56th postnatal day for control mice and mice treated with genistein. Figure 3B represents the bar graphs plotting the relative mRNA expression (y-axis) of Ppib, Gilz, Fkbp5, Sgk1, Klf13, Per1, Il13ra2, and 11bhsdII for control and genistein-treated mice at baseline (x-axis). Figure 3C represents the bar graphs plotting relative mRNA expression of Gilz, Fkbp5, Sgk1, and Klf13 (y-axis) for the vehicle- and Dex-treated control and genistein groups (x-axis). Figures 3D represents the bar graphs plotting relative mRNA expression of expression of Per1, Il13ra1, and 11bHsdII for the vehicle- and Dex-treated control and genistein groups (x-axis).

Effect of neonatal genistein exposure on glucocorticoid target gene expression in the adult uterus. (A) Schematic representation of dosing schedule, surgical intervention, and experimental end points. Body and uterine weight (g) was determined on PND56 for control and genistein-treated mice. Data represent mean of 17–21 mice±SEM. (B and C) Relative mRNA expression of Ppib, Gilz, Fkbp5, Sgk1, Klf13, Per1, 11βhsdII, and Il13ra2 as measured by qRT-PCR 4 h following intraperitoneal injection of vehicle or 1mg/kg Dex in adult ADV/OVX mice on PND56. (B) Basal expression was determined by comparing the vehicle-treated genistein group to vehicle-treated control group. (C) Fold change was determined by reporting values relative to vehicle-treated controls. Values are normalized to the reference gene Ppib. (D) Per1, 11βhsdII, and Il13ra2 mRNA expression was determined by reporting values relative to vehicle-treated controls. Bar graphs show the mean±SEM from 3 to 5 animals. * p<0.05, ** p<0.01 as determined by ANOVA with Tukey’s post hoc analysis. ADX/OVX, adrenalectomized/ovariectomized; Dex, dexamethasone; PND, postnatal day; qRT-PCR, quantitative real-time polymerase chain reaction; Treat, treatment; Veh, vehicle.