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A: Putative molecular structure of OAHFA 18:1/32:1 1 derived from meibum. B: CID mass spectrum of m/z 757.7 precursor ion. C: The OzID mass spectrum of the m/z 757.7 precursor ion provided evidence for double bond positions at n-9 and n-7 locations in both HFA and FA. Sequential CID/OzID spectra identifying carbon-carbon double bond location in HFA 32:1 chain (D) and FA 18:1 chain (E). The * indicates an artifact signal in the spectrum.
