Table 1.

Demographic and exposure characteristics of the study groups from NIEHS Clinical Research Unit and Multi-Ethnic Study of Atherosclerosis summarized by analysis group. Additional detail on the relationships between these analysis groups is available in Excel Table S1.

Characteristic	CRU-RRBS $\text{CD}14+$		MESA-RRBS $\text{CD}14+$		CRU-RRBS- 5 Celltypes		CRU-Pyro		CRU-BSAS $\text{CD}14+$		CRU-Genotyping		CRU-BSAS Saliva		$\text{CRU-CD}14+$ mRNA		CRU-eRNAa$\text{CD}14+$$\text{CD}15+$		CRU-Deep RNA seq $\text{CD}14+$
	Smoker ($n=18$)	Non smoker ($n=20$)	Smoker ($n=28$)	Non smoker ($n=27$)	Smoker ($n=9$)	Non smoker ($n=10$)	Smoker ($n=48$)	Non smoker ($n=45$)	Smoker ($n=44$)	Non smoker ($n=31$)	Smoker ($n=40$)	Non smoker ($n=43$)	Smoker ($n=37$)	Non smoker ($n=35$)	Smoker ($n=29$)	Non smoker ($n=31$)	Smoker ($n=16$)	Non smoker ($n=15$)	Smoker ($n=3$)	Non smoker ($n=3$)
Age, y [mean (SD), range]	45 (8) 27–55	44 (8) 23–53	66 (8) 55–81	67 (8) 55–80	46 (8) 32–55	46 (6) 31–53	44 (10) 18–56	40 (9) 23–53	44 (10) 18–56	40 (9) 24–53	44 (10) 18–56	39 (9) 23–53	43 (10) 18–55	39 (9) 24–53	44 (8) 23–55	41 (9) 23–53	43 (8) 27–53	44 (9) 25–53	45 (11) 34–55	43 (8) 35–50
Sex (% women)	61	60	54	56	100	100	69	69	66	70	68	67	78	71	72	74	56	60	67	67
Black (n)	9	9	6	6	5	5	27	23	24	17	24	21	18	19	15	17	8	7	1	1
White (n)	8	11	17	16	4	5	19	22	18	14	14	22	17	16	13	14	7	8	2	2
Other (n)	1b	—	5c	5c	—	—	2b	—	2b	—	2b	—	2b	—	1b	—	1b	—	—	—
Cotininec, ng/mL serum [mean (SD)]	249 (106)	2 (0)	—	—	217 (102)	2 (0)	231 (138)	2 (1)	230 (143)	2 (0)	224 (120)	2 (0)	221 (146)	2 (2)	263 (150)	2 (2)	245 (156)	2 (0)	314 (140)	2 (0)
Cotinined, ng/mL urine [mean (SD)]	—	—	5,367 (5,757)	7 (0)	—	—	—	—	—	—	—	—	—	—	—	—	—	—	—	—
Cigarettes per day [mean (SD)]	19 (9)	—	34 (25)	—	22 (11)	—	16 (9)	—	16 (9)	—	16 (9)	—	15 (9)	—	18 (8)	—	17 (5)	—	11 (5)	—
Pack-years [mean (SD)]	27 (20)	—	11 (7)	—	34 (24)	—	21 (19)	—	21 (19)	—	23 (18)	—	19 (18)	—	24 (17)	—	22 (12)	—	14 (12)	—

Note: —, not applicable; BSAS, bisulfite amplicon sequencing; CRU, clinical research unit; DNaseI, DNase I hypersensitivity region; eRNA, enhancer noncoding RNA; MESA, Multi-Ethnic Study of Atherosclerosis; NIEHS, National Institute of Environmental Health Sciences; RRBS, reduced representation bisulfite sequencing.

^aeRNA subjects are aggregated, specific assays and analyses are noted in Excel Table S1.

^bCRU Other: American Indian/Alaska Native.

^cMESA Other: Hispanic.

^cCRU Cotinine measured in serum.

^dMESA Cotinine measured in urine.