Scheme 1. Synthesis of 1-PP-Ins(4,5)P2 (1). Reagents and conditions: a. i. 5-phenyl-1H-tetrazole, 5, CH2Cl2; ii. mCPBA, CH2Cl2, 89%; b. i. DBU, BSTFA, CDCl3; ii. MeOH, then TFA; iii. 5-phenyl-1H-tetrazole, (BnO)2PNPri2, CH2Cl2; iv. mCPBA, CH2Cl2, 90%; c. i. H2, Pd(OH)2/C, MeOH, H2O, NaHCO3; ii. Ion-exchange chromatography on Q-Sepharose Fast Flow resin, 57%. Bn, benzyl.
