Extended Data Figure 6. Two conformations of the Grappler element and location of the post HSA domain.

Masked 3D classifications identified two conformations of the grappler element of INO80^core and the postHSA domain of the INO80^ATPase.

(a) Left: grappler conformation A as discussed in the main text. Right: “open” conformation B in which the bar interacts with SHL-1 of the nucleosome.

(b) Subclass showing the postHSA domain (magenta) at the INO80^ATPase (red). postHSA protrudes towards extranucleosomal DNA. Ies2 is depicted in orange.

(c) Detailed view of the map around postHSA and extranucleosomal DNA with superimposed models.

(d) Hidden Markov Model (HMM) sequence logo of Ies2, showing high sequence conservation at key Ino80 and Rvb1/2 interaction sites.