Extended Data Figure 5. Details of Rvb1/2-Ino80^insert interactions.

(a) Close-up views of Rvb1 client cavities (blue), bound to the different interaction elements of Ino80^insert (red, with yellow hydrophobic and green aromatic side chains).

(b) Same as (a), but depicting Rvb2 client cavities

(c) Ino80^insert shown in rainbow coloring (red: N-terminus, blue C-terminus) to highlight the circular fold. Selected elements as well as the positions of the Rvb1/2 binding partners are annotated.

(d) Same as (c) but viewed from the side to highlight the protruding “plug” and “latch” elements

(e) Rvb1/2 pair (pair “c” from the hexamer in Fig. 1c) bound to Ino80^insert in comparison with

(f) a Rvb1/2 pair from the unliganded dodecameric state (PDB: 4wvy). The comparison shows how client binding arranges the AAA+, OB and middle layers and displaces the N-terminal domain of Rvb1 from the client pocket, seen also for hINO80^{core 23}. Both types of conformational changes impact on the ADP binding site (ADP, ATP: color coded spheres), suggesting how client interactions are allosterically coupled to ATPase activity of Rvb1/2.

(g) Exemplary view of the ADP coordination along with the superimposed map.