Fig. 4. RAW264.7 macrophage viability after LPS stimulation. Macrophages (1 × 104 cells per well) were seeded in a 96-well plate, treated with PLNPs (125 μg lipid per ml) and LPS (1 μg ml–1) and incubated for 48 h. Absorbance was measured at 450 nm with a microplate reader at the indicated times. Data are the means ± S.D. ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001.
