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. 2018 Aug 2;13(8):e0200214. doi: 10.1371/journal.pone.0200214

Table 1. Geographical information and genetic diversity for Calotriton asper populations.

Geographical information and estimates of genetic diversity parameters for each Calotriton asper population and cluster defined by STRUCTURE analysis. Altitude in meters; N, sample size; Na: number of alleles per locus; Ar, allelic richness; PA, number of private alleles; PAAr, allelic richness of private alleles; FIS, inbreeding coefficient; HO, observed heterozygosity; HE, expected heterozygosity. Values in bold indicate statistical significance after Bonferroni’s correction.

Grouping Code Long Lat Alt Habitat N Na Ar PA PAAr HO HE FIS
Population                          
Irati Spain ISP -1.14 43.01 848 Stream 16 4.895 3.210 1 0.020 0.576 0.596 0.067
Irati France IFR -1.06 43.05 1105 Stream 15 4.421 3.080   0.010 0.603 0.572 -0.019
Olhadoko OLH -0.95 42.99 662 Stream 15 4.474 3.050   0.010 0.630 0.572 -0.068
Barranco Gamuetta BGA -0.80 42.89 1324 Stream 50 7.000 3.500   0.030 0.609 0.633 0.049
Linza LIN -0.80 42.90 1374 Stream 39 6.632 3.490 1 0.030 0.606 0.621 0.037
Valdagras VAL -0.79 42.86 1266 Stream 39 6.000 3.400   0.020 0.641 0.619 -0.022
Barranco de Petraficha BPE -0.77 42.86 1427 Stream 21 6.263 3.680   0.020 0.644 0.650 0.034
Selva de Oza SOZ -0.71 42.83 1181 Stream 21 5.789 3.590   0.010 0.642 0.656 0.047
Barranco de Acherito BAC -0.71 42.87 1385 Stream 6 4.158 3.540   0.010 0.675 0.615 -0.007
Ansabere ANS -0.71 42.89 1787 Stream 20 4.368 3.020   0.010 0.579 0.573 0.016
Ibón de Acherito IAC -0.71 42.88 1882 Lake 44 6.316 3.260 1 0.030 0.595 0.598 0.017
Riglos RIG -0.70 42.34 920 Stream 4 3.000 3.000   0.100 0.461 0.487 0.219
Ibón de Saman ISA -0.48 42.74 2143 Lake 20 4.421 2.900 1 0.060 0.535 0.539 0.034
Ibón de Bucuesa IBU -0.43 42.71 2126 Lake 19 3.158 2.440 1 0.090 0.460 0.449 0.002
Ibón de Espeluchieca IES -0.43 42.79 1966 Lake 20 4.526 2.930   0.000 0.563 0.528 -0.040
Montrepos MON -0.39 42.34 1220 Stream 26 7.947 4.450 10 0.400 0.691 0.777 0.131
Sierra de Guara SGU -0.25 42.26 975 Stream 18 6.368 3.920 7 0.380 0.684 0.689 0.036
Betharram BET -0.19 43.10 451 Cave 31 1.579 1.440   0.000 0.182 0.182 0.016
Genie Longue GLO -0.15 43.05 671 Stream 16 3.368 2.280   0.000 0.408 0.388 -0.020
Oto OTO -0.15 42.59 1137 Stream 18 7.263 4.120 4 0.180 0.712 0.711 0.028
Sarvisé SAR -0.07 42.58 1359 Stream 21 8.211 4.500 4 0.230 0.723 0.764 0.078
Barbarisa BAR 0.41 42.61 2333 Lake 12 4.684 3.360   0.090 0.605 0.609 0.049
Barranco de Sabaril BSA 0.42 42.58 1684 Stream 20 5.316 3.280 1 0.040 0.597 0.601 0.032
Ibón de Perramó IPE 0.50 42.64 2406 Lake 69 4.053 2.510   0.010 0.448 0.457 0.028
Barranco de Batisielles BBA 0.51 42.67 1815 Stream 6 3.737 3.270   0.020 0.553 0.564 0.111
Barranco del Pino BPI 0.52 42.66 1604 Stream 15 4.421 3.080   0.010 0.544 0.569 0.078
Ibón de Alba IAL 0.61 42.66 2301 Lake 64 4.684 2.690 3 0.080 0.495 0.505 0.028
Pas du Loup PDL 1.00 43.01 489 Cave 18 1.737 1.480   0.000 0.184 0.179 -0.001
Vilanova de Meià VME 1.03 42.02 847 Stream 19 3.368 2.500 1 0.120 0.483 0.452 -0.039
Font Bordonera FBO 1.30 42.20 748 Stream 20 3.632 2.330   0.010 0.387 0.386 0.023
Arcouzan ARC 1.12 42.80 1214 Stream 8 3.895 3.280   0.000 0.704 0.620 -0.069
Ribaui RIB 1.34 42.79 802 Stream 13 4.316 3.200   0.030 0.636 0.600 -0.019
Courbiere COU 1.45 42.85 1626 Stream 6 3.211 2.860   0.000 0.579 0.542 0.024
Vicdessos VIC 1.49 42.77 725 Cave 7 3.158 2.650 1 0.030 0.549 0.480 -0.068
Siech SIE 1.55 42.88 691 Cave 8 2.421 2.150 1 0.030 0.375 0.380 0.079
Bernard BER 1.53 43.00 565 Cave 26 2.579 1.990   0.000 0.366 0.353 -0.019
Labouiche LAB 1.57 43.00 485 Cave 17 2.947 2.420   0.010 0.483 0.468 0.000
Cailla CAI 2.19 42.81 724 Stream 23 3.000 2.190   0.010 0.455 0.409 -0.030
Cass Rats CRA 2.32 42.88 522 Stream 22 1.947 1.660   0.000 0.282 0.245 -0.127
Font de Dotz FDD 2.36 42.88 485 Cave 4 1.842 1.840 1 0.050 0.434 0.289 -0.385
Auriac AUR 2.49 42.93 543 Stream 7 1.789 1.700   0.000 0.293 0.269 -0.013
Vidrà VID 2.34 42.13 1068 Stream 14 6.526 4.030   0.040 0.473 0.710 0.367
St Pau de Segúries SSE 2.38 42.27 892 Stream 13 2.684 2.110   0.030 0.356 0.354 0.034
Valmanya VAM 2.54 42.53 924 Stream 10 2.421 2.000   0.000 0.358 0.318 -0.075
Clusters                          
Cluster 1           479 13.842 13.700 72 5.998 0.582 0.774 0.250
Cluster 1.1           193 13.000 11.670 48 2.240 0.534 0.759 0.299
Cluster 1.2           286 9.316 7.880 8 0.300 0.614 0.685 0.105
Cluster 2           421 11.158 11.160 21 0.002 0.452 0.737 0.388
Cluster 2.1           204 7.842 6.790 9 0.400 0.473 0.581 0.188
Cluster 2.2           76 7.842 8.470 3 0.250 0.518 0.726 0.352
Cluster 2.3           141 8.579 6.010 1 0.130 0.383 0.658 0.276