Fig 1. Experimental design of the transgenerational zebrafish feeding trial.

F₀ zebrafish from both control and high ARA group were fed a start feed containing Gemma micro^® and Artemia nauplii from 5 DPF until 26 DPF. The two experimental groups were given either a control or high ARA diet from 27 DPF onwards until sampling. F₀ fish were mated at 97 DPF to produce F₁ generation. Both groups in the F₁ generation were fed the control diet from 27 DPF until sampling. F₀ and F₁ body weight (grams) and liver tissue sampling for transcriptome analysis (RNA-sequencing) were performed at 154–156 DPF (F₀) and 140–142 DPF (F₁).