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. 2018 May 18;126(5):057005. doi: 10.1289/EHP2387

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EHP is an open-access journal published with support from the National Institute of Environmental Health Sciences, National Institutes of Health. All content is public domain unless otherwise noted.

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Figures 3A, 3B, 3C, and 3D are bar graphs plotting the change in F340/380 (y-axis) across E2, 4 prime OH BDE 049, 5 prime OH BDE 099, and 3 prime OH BDE 154, respectively, (x-axis) measured in control group, 0.1, 1, 10, 100, and 1000 nanomolar for E2, and measured in control group, 10, 100, 1000 and 10000 nanomolar for 4 prime OH BDE 049, 5 prime OH BDE 099, and 3 prime OH BDE 154. Inset each figure subpart are line graphs plotting the same across time in seconds. Figure 3E is a bar graph plotting the relative value of F340/380 (y-axis) across control group, E2, and 11 OH BDEs (x-axis) in the presence of 10 micromolar G15 and in the absence of G15. — Effects of $17 beta estradiol$ ( $E sub 2$ ) and 11 hydroxylated polybrominated diphenyl ethers (OH-PBDEs) on calcium mobilization in SKBR3 cells and the inhibitory effects of G15. (A–D) Calcium mobilization induced by different concentrations of $E sub 2$ , 4ʹ-OH-BDE-049, 5ʹ-OH-BDE-099, and 3ʹ-OH-BDE-154. The insets denote the change of F340/380 (the ratio of fluorescence intensity at $340 nanometers$ and $380 nanometers$ of calcium indicator fura-2-acetoxymethyl ester) with time, and the arrows indicate the time at which the compounds were added. (E) Calcium mobilization induced by $100 nanomolar$ $E sub 2$ and $1 micromolar$ OH-PBDEs in the absence or presence of $10 micromolar$ G15, a G protein–coupled estrogen receptor antagonist. The relative value of F340/380 after (the average of the last three data points in the time course curve as shown in the insets of A–D) and before adding the compounds is used to characterize the calcium mobilization. * $p less than 0.05$ compared with the control group (Ctrl, 0.1% dimethyl sulfoxide). # $p less than 0.05$ compared with the groups treated with compounds in the absence of G15.