Figure 4.

Effects of $17\mathrm{\beta }\text{-estradiol}$ (${\mathrm{E}}_2$) and 11 hydroxylated polybrominated diphenyl ethers (OH-PBDEs) on cyclic adenosine monophosphate (cAMP) production in SKBR3 cells and the inhibitory effects of G15. (A) cAMP production induced by different concentrations of ${\mathrm{E}}_2$ (0, $0.1\text{nM}$, $1\text{nM}$, $10\text{nM}$, $100\text{nM}$, and $1\mathrm{\mu }\mathrm{M}$) and OH-PBDEs (0, $1\text{nM}$, $10\text{nM}$, $100\text{nM}$, $1\mathrm{\mu }\mathrm{M}$, and $10\mathrm{\mu }\mathrm{M}$). (B) cAMP production induced by $100\text{nM}$ ${\mathrm{E}}_2$ and $1\mathrm{\mu }\mathrm{M}$ OH-PBDEs in the absence or presence of $10\mathrm{\mu }\mathrm{M}$ G15, a G protein–coupled estrogen receptor antagonist. *$p<0.05$ compared with the control group (Ctrl, 0.1% dimethyl sulfoxide). #$p<0.05$ compared with the groups treated with compounds in the absence of G15.