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. 2018 May 18;126(5):057005. doi: 10.1289/EHP2387

Figure 6.

Figure 6A is a bar graph plotting the percentage of relative migrated cells (y-axis) across concentrations of E2 in 0 to 1 micromolar, and 4 prime OH BDE 049, 5 prime OH BDE 099, and 3 prime OH BDE 154 (x-axis), all measured in 0 to 10 micromolar. Figure 6B presents micrographs of the Boyden chamber assay for the control group, 1 micromolar 5 prime OH BDE 099 and 1 micromolar 5 prime OH BDE 099 plus 10 micromolar G15. Figure 6C is a bar graph plotting the percentage of relative migrated cells (y-axis) across control group, 100 nanomolar E2, 1 micromolar 4 prime OH BDE 049, 1 micromolar 5 prime OH BDE 099, and 1 micromolar 3 prime OH BDE 154 (x-axis) in the presence of 10 micromolar G15 and in the absence of G15.

Effects of 17β-estradiol (E2) and three hydroxylated polybrominated diphenyl ethers (OH-PBDEs) on SKBR3 cell migration detected by Boyden chamber assay and the inhibitory effects of G15. (A) SKBR3 cell migration induced by different concentrations of E2 (0, 0.1 nM, 1 nM, 10 nM, 100 nM, and 1μM) and OH-PBDEs (0, 1 nM, 10 nM, 100 nM, 1μM, and 10μM). (B) A typical Boyden chamber assay result for 1μM 5ʹ-OH-BDE-099 in the absence or presence of 10μM G15, a G protein–coupled estrogen receptor antagonist. (C) SKBR3 cell migration induced by 100 nM E2 and 1μM OH-PBDEs in the absence or presence of 10μM G15. The relative migration of cells is calculated by setting the count of migrated cells of the control group (Ctrl) as 100%. *p<0.05 compared with the control group (Ctrl, 0.1% dimethyl sulfoxide). #p<0.05 compared with the groups treated with compounds in absence of G15.