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. 2018 May 21;126(5):057006. doi: 10.1289/EHP3030

Figure 5.

Figures 5A and 5B each comprise two graphs each plotting the concentration of glucose in milligrams per deciliter and area under the curve during glucose tolerance (y-axis) for male and female animals, respectively, from set 1, and treated with DMSO, TBT, 50 nanomolar DBT, and 500 nanomolar DBT. Glucose levels are measured from 0 till 120 minutes (x-axis). Figures 5C and 5D each comprise two graphs each plotting the concentration of glucose in milligrams per deciliter and area under the curve during insulin tolerance test (y-axis) for male and female animals, respectively, and in utero exposed to DMSO, TBT, 50 nanomolar DBT, and 500 nanomolar DBT. Glucose levels are measured from 0 till 120 minutes (x-axis).

DBT effects on glucose homeostasis in males and females from set 1. Glucose levels and area under the curve (AUC) during glucose tolerance (A, B) and insulin tolerance (C, D) tests in males and females. Animals were fasted for 4 h prior to the test. Glucose or insulin was administered by intraperitoneal injection and glucose levels were measured every 30 min for the next 120 min. AUC was calculated for each animal independently and averaged for each treatment group. Two-way analysis of variance (ANOVA) was conducted to compare DMSO and the different treatments, followed by Sidak’s post-hoc test for glucose levels at each time point. One-way ANOVA was conducted to compare DMSO and the different concentrations of DBT, followed by Dunnett’s post-hoc test to compare differences in AUC. Unpaired t-test was conducted for TBT versus vehicle. N ≧ 6. Data are expressed as the mean±SEM. Note: DBT, dibutyltin; DMSO, dimethylsulfoxide; SEM,standard error of the mean; TBT, tributyltin.