Table 1. InDel variants at the canonical GP editing site.
Huh7 and Mpg cells were infected with EBOV, SUDV, BDBV, or RESTV at moi = 0.1. Total RNA was harvested 3 dpi, and purified mRNAs were used to make NGS libraries. Variant detection was done using a minimum cut-off of 1%.
| InDel variants at GP editing site | |||||
|---|---|---|---|---|---|
| Reference | Predicted | Frequency (%) | |||
| sequence | Position | Allele | protein | Huh7 | Mpg |
| EBOV | 6918 | 7A | sGP | 66.8 | 61.4 |
| 8A | GP | 19.5 | 19.1 | ||
| 9A | ssGP | 1.5 | 2.3 | ||
| SUDV | 6877 | 7A | sGP | 56.1 | 57.1 |
| 8A | GP | 17.2 | 20.6 | ||
| 9A | ssGP | 2.3 | 2.6 | ||
| 10A | sGP+1aa | 2.8 | 1.9 | ||
| 11A | GP+1aa | 1.2 | − | ||
| 12A | ssGP+1aa | − | 1.1 | ||
| BDBV | 6900 | 7A | sGP | 69.5 | 62.1 |
| 8A | GP | 19.2 | 23.3 | ||
| 9A | ssGP | 1.4 | 2.3 | ||
| 10A | sGP+1aa | − | − | ||
| 11A | GP+1aa | − | 1.1 | ||
| RESTV | 6912 | 7A | sGP | 78.1 | 71.8 |
| 8A | GP | 14.4 | 16.8 | ||
| 9A | ssGP | 3.0 | 3.0 | ||
| 10A | sGP+1aa | − | 1.4 | ||