Figure 4.

DLX5 was a direct target of miR-339-5p. (A) The construction profile of the pLUC-DLX5 vector containing the miR-339-5p target sites in DLX5 3′UTR. Relative luciferase activity of HFSCs that were co-transfected with pLUC plasmid and miR-339-5p mimic, inhibitor, or corresponding controls. (B) DLX5 expression was measured in transfected HFSCs. (C) Representative images of DLX5 immunofluorescence staining of transfected HFSCs. Red indicates DLX5 fluorescence, and blue indicates the DAPI counterstain. Scale bar, 25 µm. *P<0.05 vs. control group. DLX5, homeobox protein DLX-5; miR-339-5p, microRNA-339-5p; HFSC, hair follicle stem cells; NC, negative control.