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. 2018 Jun 5;18(2):1616–1622. doi: 10.3892/mmr.2018.9131

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — AFAP1-AS1 promoted OS cell proliferation and invasion by regulating activation of TCF4/β-catenin pathway. (A) RT-qPCR was used to determine the expression of TCF4 in 58 paired OS tissues and adjacent normal tissues. (B) RT-qPCR analysis indicated TCF4 expression was significantly upregulated after transfection and western blot indicated that ectopic expression of TCF4 restored the TCF4 protein level and Wnt/β-catenin pathway activation in Saos-2 and U2OS cells transfected with siAFAP1-AS1 or miR-4695-5p mimics. (C) CCK-8 assays were used to measure the proliferation of Saos-2 and U2OS cells. (D) Transwell assays were utilized to determine the invasion of Saos-2 and U2OS cells. The results represented three independent experiments and were expressed as mean ± SD. **P<0.01 vs. the control group. TCF4, transcription factor 4; OS, osteosarcoma; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; CCK-8, Cell Counting Kit-8.