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. 2018 May 29;18(2):1628–1636. doi: 10.3892/mmr.2018.9099

Figure 5.

Figure 5.

Smad3 is crucial to cell proliferation and metastasis of HKF cells. (A) Effects of miR-637 siRNA and Smad3 siRNA on cell proliferation ability using MTT. Data are presented as mean ± SEM. **P<0.01, ^^P<0.01 ##P<0.01 as indicated. (B) Effects of miR-637 siRNA and Smad3 siRNA on cell proliferation ability using transwell assay. Cells were counted and results represent the mean ± SD of three experiments (magnification, ×200). **P<0.01, ^^P<0.01 ##P<0.01 as indicated. (C and D) Effects of miR-637 siRNA and Smad3 siRNA on Smad3, Cyclin D1 and MMP2 were detected by western blotting and qPCR. Data are shown as mean ± SEM. HKF, human keloid fibroblast; MMP, Matrix Metallopeptidase; qPCR, quantitative polymerase chain reaction; Smad, mothers against decapentaplegic homolog; SEM, standard error of the mean. **P<0.01, ^^P<0.01 ##P<0.01 as indicated.