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Effect of tensile stress on protein expression in Saos-2 cells. (A) p-GSK-3β and GSK-3β protein expression in cytosolic fractions was determined by western blot analysis following 1 h of 0 or 12% tensile stress. (B) Expression level of nuclear β-catenin protein was determined by western blot analysis following 1 h of 0 or 12% tensile stress. p-, phosphorylated; GSK, glycogen synthase kinase.
