Table I.
Primer sequences used in semi-quantitative reverse transcription-polymerase chain reaction.
Primer | ||||
---|---|---|---|---|
Gene | Forward | Reverse | Size, bp | Annealing temperature, °C |
β-actin | 5′-ATCATGTTTGAGACCTTCAA-3′ | 5′-CATCTCTTGCTCGAAGTCCA-3′ | 212 | 55 |
β-catenin | 5′-AAGGTCTGAGGAGCAGCTTC-3′ | 5′-TGGACCATAACTGCAGCCTT-3′ | 668 | 55 |
COX-2 | 5′-TCAAGTCCCTGAGCATCTAC-3′ | 5′-CATTCCTACCACCAGCAACC-3′ | 488 | 58 |
Cyclin D1 | 5′-GAGACCATCCCCCTGACGGC-3′ | 5′-CTCTTCCTCCTCCTCGGCGGC-3′ | 484 | 56 |
c-fos | 5′-ACCAGTCCGGACCTGCAGTGG-3′ | 5′-GCGGCATTTGGCTGCAGCCAT-3′ | 261 | 57 |
c-Jun | 5′-CCCCTGTCCCCCATCGACATG-3′ | 5′-TTGCAACTGCGTTAGCAT-3′ | 267 | 55 |
COX, cyclooxygenase.