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. 2018 Jun 20;18(2):1947–1954. doi: 10.3892/mmr.2018.9197

Figure 4.

Figure 4.

Cell apoptosis of renal tubular epithelial cells in the control, empty and HBx groups was evaluated by Annexin V-FITC/PI double staining and subsequent flow cytometry. Cells transfected with empty plasmid pcDNA3.1(+) and recombinant plasmid pcDNA3.1(+)-HBx were designated as the empty and HBx groups, respectively. Cells without transfection served as the control group. Q1-2 and Q1-4 quadrants represent cells undergoing apoptosis. *P<0.05 vs. empty group. HBx, hepatitis B virus X protein; FITC, fluorescein isothiocyanate; PI, propidium iodide.