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. 2018 Jun 20;18(2):1987–1994. doi: 10.3892/mmr.2018.9195

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Overexpression of KLF15 regulates endothelial cell associated factors in Eahy926 cells. (A) mRNA expression levels of eNOS, MCP-1, ICAM-1 and TGF-β1 were determined via reverse transcription-quantitative polymerase chain reaction. (B) The protein expression levels of eNOS, MCP-1, ICAM-1 and TGF-β1 were investigated via western blotting. β-actin was used as an internal control. *P<0.05, **P<0.01 and ***P<0.001 vs. respective NC. ^{^}P<0.05, ^{^^}P<0.01 and ^{^^^}P<0.001 vs. respective TNF-α + NC. NC, negative control pTA2 vector; KLF15, Krüppel-like factor 15; TNF-α, tumor necrosis factor-α; eNOS, endothelial nitric oxide synthase; MCP-1, monocyte chemoattractant protein-1; ICAM-1, intercellular adhesion molecule-1; TGF-β1, transforming growth factor-β1.