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Luciferase and western blot analysis indicated ATG12 to be the target of miR-23a. (A) Predicted binding site for miR-23a in 3′UTR of ATG12 mRNA, and the mutated sequence. (B) Luciferase activity comparison between ATG12-wt and ATG12-mut plasmid. (C) Western blot analysis of ATG12 in the negative control, miR-23a mimics and miR-23a inhibition groups. **P<0.01. ATG12, ubiquitin-like protein ATG12; miR, miRNA; UTR, untranslated region; wt, wild-type; mut, mutant.
