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. 2018 Jun 25;18(2):2198–2206. doi: 10.3892/mmr.2018.9216

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Copyright: © Pang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Effects of rCC16 on TNF-α, IL-6 and IL-8 expression in CS-exposed mice. The expression of TNF-α, IL-6 and IL-8 (A-C) mRNA and (D-F) protein in CS-exposed mice treated with rCC16. mice were exposed to CS for 24 weeks and treated intranasally with rCC16 for the last 4 weeks. For qPCR measurements, the results are expressed as ratios of 2^−∆∆Ct values for TNF-α, IL-6 and IL-8 mRNA/β-actin. For ELISA measurements, results are expressed as the absolute protein values of TNF-α, IL-6 and IL-8. Data are presented as the mean ± SD (n=5/group). *P<0.05 and **P<0.01 as indicated. BALF, bronchoalveolar lavage fluid; CS, cigarette smoke; rCC16, recombinant club cell secretory protein; SD, standard deviation.