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. 2018 Jun 22;18(2):2124–2132. doi: 10.3892/mmr.2018.9209

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Copyright: © Yu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — 3-MA significantly increased the apoptotic rate under hypoxic conditions. Apoptosis was detected by a Annexin V-fluorescein isothiocyanate assay. (A) Flow cytometry analysis of apoptosis. Q2 represented the early apoptotic cells. Q4 represented the late apoptotic cells. Apoptotic cells from each group were detected by Annexin V and propidium iodide. (B) The proportion of apoptotic cells from each group was quantified. Data are presented as the mean ± standard deviation. *P<0.05 vs. control group, &P<0.05 vs. CoCl2 group. Q, quadrant; CoCl2, cobalt (II) chloride; 3-MA, 3-methyladenine.