Table I.
Sequences of primers used for reverse transcription-quantitative polymerase chain reaction.
| Primer | Sequences (5′-3′) | Fragment length (bp) | Annealing temperature (°C) |
|---|---|---|---|
| ALB | F: TGCTTGAATGTGCTGATGACAGGG | 162 | 60 |
| R: AAGGCAAGTCAGCAGGCATCTCATC | |||
| AFP | F: GAAACCCACTGGAGATGAACAGTC | 190 | 60 |
| R: AAGTGGGATCGATGCAGGA | |||
| TAT | F: TGAGCAGTCTGTCCACTGCCT | 359 | 60 |
| R: ATGTGAATGAGGAGGATCTGAG | |||
| G-6P | F: GCTGGAGTCCTGTCAGGCATTGC | 349 | 60 |
| R: TAGAGCTGAGGCGGAATGGGAG | |||
| CYP3A4 | F: TGTGCCTGAGAACACCAGAG | 202 | 60 |
| R: GCAGAGGAGCCAAATCTACC | |||
| α1AT | F: CTGGGACAGTGAATCGACAATGC | 560 | 54 |
| R: TCTGTTTCTTGGCCTCTTGGTG | |||
| GAPDH | F: AGAAGGCTGGGGCTCATTTG | 258 | 52 |
| R: AGGGCCATCCACAGTCTTC |
ALB, albumin; CYP3A4, cytochrome P450 3A4; TAT, tyrosine-aminotransferase; G-6P, glucose-6phosphate; α1AT, α1 antitrypsin; AFP, α-fetoprotein; F, forward; R, reverse.