Skip to main content
. 2018 Jul 18;3(7):8042–8049. doi: 10.1021/acsomega.8b00738

Figure 4.

Figure 4

(a) Cell viability on the treatment of HeLa cells with siRNA-loaded nanogels and incubation for 36 h post-transfection. (b) Flow cytometry analysis of apoptosis induced by knockdown of PLK1 gene, by siRNA-loaded nanogels, 24 h post-transfection. The error bars represent the percent error obtained from multiple experiments. (c) PLK1 gene expression 24 h post-transfection of siRNA-loaded pNIPAm and p(NIPMAm-co-NIPAm) nanogels by real-time polymerase chain reaction (RT-PCR). Error bars indicate standard deviation between triplicates. Lipo: lipofectamine loaded with 75 nM PLK1 siRNA, Scr: nanogel with 75 nM scrambled siRNA, and UC: untreated cells. Error bars indicate standard deviation between multiple experiments. *P < 0.05. Note: p(NIPMAm-co-NIPAm)–PεL is denoted as pNIPMAm–PεL.