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. Author manuscript; available in PMC: 2018 Aug 2.
Published in final edited form as: Cell Rep. 2018 Jun 5;23(10):3031–3041. doi: 10.1016/j.celrep.2018.05.020

Figure 1. Multiple Pyrimidine Analogs Are Successfully Incorporated by Telomerase.

Figure 1

(A) A series of non-native pyrimidine analogs were tested for incorporation into telomere ssDNA by telomerase using an in vitro direct extension assay. The structures and electron density surface potentials of the analogs are indicated. Electronegative regions are indicated in red, neutral regions are indicated in green, and the electropositive regions are indicated in blue.

(B) A telomerase extension assay reveals extended products. LC, loading control. Numbers on the left display the number of hexamer repeats added by telomerase. Numbers at the bottom of telomerase assay correlate with the compounds that were used in place of dTTP in the telomerase extension assay, as labeled in (A).

(C) Extended products were analyzed to quantify the telomerase activity and repeat addition processivity relative to that of native dTTP. Data are represented as mean ± SEM (n = 3).