Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jun 12;40(2):609–620. doi: 10.3892/or.2018.6486

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Jin et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 6. — Norcantharidin (NCTD) sensitizes non-small cell lung cancer (NSCLC) cells to cisplatin (DDP) and suppresses YAP-mediated invasiveness and epithelial-mesenchymal transition (EMT). (A) RT-PCR and western blot analysis showing the mRNA and protein expression of E-cadherin and vimentin in A549/DDP cells transfected with siRNA or YAP overexpression vector. (B and C) RT-qPCR and western blot analysis and densitometric quantification demonstrating that NCTD enhanced the DDP-induced increase or decrease in the (B) mRNA and (C) protein level of E-cadherin or vimentin in A549/DDP cells. (D) Scratch assay showing that NCTD enhanced the DDP-induced decrease in the migration of A549/DDP cells at 36 h. (E) Transwell assay identified that NCTD enhanced the DDP-induced decrease in cell invasive growth and migration of the A549/DDP cells. (F) The correlation between the co-treatment with NCTD and DDP and EMT marker protein levels, E-cadherin and vimentin, was analyzed based on the results of western blot analysis, respectively. Results are presented as the means ± SD, and the error bars represent the SD of 3 independent experiments. ***P<0.001 vs. the control group. NS, not significant.