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. 2018 May 24;40(2):693–703. doi: 10.3892/or.2018.6464

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Copyright: © Tamura et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — NANOG regulates colorectal cancer cell proliferation through the control of cyclin D1 expression. (A) Growth curve of HCT116 colorectal cancer cells. NANOG siRNA-transfected cells exhibited decreased cell proliferation compared to the control. (B) Quantitative RT-PCR (*P<0.01) and (C) RT-PCR identified NANOG suppression in siRNA-transfected cells. (D) An immunofluorescence study revealed the downregulation of NANOG protein after siRNA transfection. (E) RT-PCR for cyclins. Cyclin D1 and cyclin B1 mRNA levels were decreased in siRNA-transfected cells, but the expression levels of other cyclins, p21, and p27 were not changed.