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Subcellular localization of SPAG4 and its fragments in A549 cells. (A) Schematic diagram of the three domains of the SPAG4 protein. Different recombinational fragments of SPAG4 were designed to study its function. (B) Plasmids of different SPAG4 fragments were electrically transfected into A549 cells, and the fluorescence distribution was observed under a confocal laser scanning microscope (magnification, ×600). SPAG4, sperm-associated antigen 4.
