Fig. 4. Phosphorylation of Stat3 in Tu-2449 cells increases with cell density. Tu-2449 glioma cells were plated with different cell numbers. (A) Cell density was microscopically monitored after 24 h (20% low, 100% high cell density), magnification: 40×, and (B) protein lysates were analyzed for phosphorylated Stat3 (P-Stat3 Y705) and total Stat3 by Western blotting analyses. Actin served as the loading control. The extent of P-Stat3 increases with cell density.
