Fig. 7. EVP4593 induces H₂O₂ emission and mitochondrial permeability transition (mPT) in rat heart mitochondria. (A) Equimolar amounts (1 μM) of EVP4593 and DQA had similar effects on the mean H₂O₂ emission over a period of 20 min. (B) Kinetics course of H₂O₂ emission (mean values of triplicates and standard deviations of one representative preparation are shown). In presence of 50 μM Ca²⁺, the EVP4593 induced H₂O₂ emission was dynamically increased over time, implicating mPT as supported by the suppressing effect of cyclosporine A (CsA).