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. 2018 Jun 25;40(3):1233–1242. doi: 10.3892/or.2018.6522

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — RAD54B mRNA and protein expression are inhibited following infection with shRAD54B in BEL-7404 and SMMC-7721 cells. (A and B) Infection efficiency of lentivirus-shRAD54B (shRAD54B) and lentivirus-shCtrl (shCtrl) in the human hepatoma cell lines BEL-7404 and SMMC-7721. Representative fluorescence images after 72 h infection are shown (magnification, ×100). (C and D) The expression of RAD54B mRNA was analyzed by RT-qPCR after infection with shRAD54B and shCtrl in BEL-7404 and SMMC-7721 cells (n=3, **P<0.01). (E-H) The protein expression of RAD54B was analyzed by western blotting after infection with shRAD54B and shCtrl in BEL-7404 and SMMC-7721 cells and quantification of the results was performed (n=3, **P<0.01).