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. 2018 Jun 26;40(3):1251–1260. doi: 10.3892/or.2018.6525

Figure 3.

Figure 3.

TRIM58 overexpression has no significant influence on the proliferation, colonization or migration of human colorectal cancer cell lines. (A-C) The overexpression of TRIM58 mRNA (A: HCT8 cells and B: KM12 cells) and (C) protein in CRC cell lines was confirmed by RT-PCR and western blot analysis.**: n=3, P<0.001 and ***: n=3, P<0.0001, two-tailed Student's t-test. (D and E) Proliferation assay (D: HCT8 cells and E: KM12 cells) conducted using IncuCyte. Cells were transfected with the TRIM58 overexpression vector on day 1 and day 3. n.s. indicates not significant, n=3, P>0.05, two-tailed Student's t-test. (F-H) Images (F) and statistical analysis (G: HCT8 cells and H: KM12 cells) of colony formation assays indicated that TRIM58 overexpression had no significant influence on cell colonization. n.s. indicates not significant, n=3, P>0.05, two-tailed Student's t-test. (I-K) Images of wound-healing assays (I) and statistical analysis (J: HCT8 cells and K: KM12 cells) were performed to show that TRIM58 did not inhibit cell migration. n.s. indicates not significant, n=3, P>0.05, two-tailed Student's t-test. Scale bar, 100 µm. TRIM, tripartite motif protein; CRC, colorectal cancer.