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. 2018 Jun 27;40(3):1339–1347. doi: 10.3892/or.2018.6531

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — OP-B inhibits tubule formation by endothelial cells. (A) Matrigel assay analysis was used to detect microtubule formation by EA.hy926 cells after treatment with 0, 5, or 10 µmol/l OP-B for 0, 2, or 4 h. Representative images are displayed (magnification, ×200). (B and C) A549 cells were treated with 0, 5 or 10 µmol/l OP-B for 24 h, and the phosphorylation levels of VEGFR2, Tie-2, p-Akt (Ser473), p-PLCγ1 (Ser1248), EphA2, and p-EphA2 (Ser897) were detected by western blotting. β-actin was used as a loading control. The experiment was repeated three times and yielded similar results. OP-B, Ophiopogonin B.