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. 2018 Jul 12;40(3):1390–1400. doi: 10.3892/or.2018.6568

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Copyright: © Lin et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Effect of tetrandrine on caspase-3 activation with or without radiation in CT26/tk-luc cells. (A) Cells were treated with 20 µM tetrandrine for 3 h with or without 4 Gy irradiation, then they were incubated for a further 3, 9 and 21 h, followed by western blot analysis for both pro- and cleaved caspase-3. A 10-column gel was used to perform the experiment. In order to include the markers, it was necessary to use two separate gels. The dotted line between lanes 7 and 8 indicates that the result was obtained from two separated gels with the samples from the same experiment. (B) CT26/tk-luc cells were treated with 0, 10, 20 and 50 µM tetrandrine with or without 4 Gy for 24 h followed by western blot analysis and the results were quantified. The activation of caspase-3 was compared between TET alone and the combination treatment groups. Camptothecin (CAM; 50 µM) was used as the positive control (P.C.). Data are presented as the mean ± standard error (**P<0.01).

Figure 3. — Effect of tetrandrine on caspase-3 activation with or without radiation in CT26/tk-luc cells. (A) Cells were treated with 20 µM tetrandrine for 3 h with or without 4 Gy irradiation, then they were incubated for a further 3, 9 and 21 h, followed by western blot analysis for both pro- and cleaved caspase-3. A 10-column gel was used to perform the experiment. In order to include the markers, it was necessary to use two separate gels. The dotted line between lanes 7 and 8 indicates that the result was obtained from two separated gels with the samples from the same experiment. (B) CT26/tk-luc cells were treated with 0, 10, 20 and 50 µM tetrandrine with or without 4 Gy for 24 h followed by western blot analysis and the results were quantified. The activation of caspase-3 was compared between TET alone and the combination treatment groups. Camptothecin (CAM; 50 µM) was used as the positive control (P.C.). Data are presented as the mean ± standard error (**P<0.01).