Fig. 2. Tau aggregation assay using fluorescence emission of thioflavin T and trapping of aggregated tau. (a) 4R refers to tau protein, in the absence of an aggregation inhibitor. A lower intensity than the standard 4R correlates to a lower concentration of ordered β-sheet structures, indicative of inhibition of tau aggregation. (b) Aggregated tau trapped and detected on membranes. Lower spot intensity is consistent with decreased amounts of fibrillar tau aggregates. Quantification of 7 independent experiments (*p < 0.05, ****p < 0.0001).
