Table 1. Statistics of the hEXO-10cat-hMPP6 cryo-EM atomic model.
| hEXO-10cat-hMPP6 | |
|---|---|
| Data collection | |
| Microscope | Titan Krios |
| Camera | Gatan K2 Summit |
| Magnification | 105,000 x |
| Voltage (kV) | 300 |
| Electron dose (e-/Å2) | 46.9 |
| Dose rate (e-/pixel/s) | 8.55 |
| Defocus range (μm) | 0.5–3.5 |
| Pixel size | 1.35 |
| Reconstruction | |
| Micrographs collected | 8047 |
| Particles in 3D classification | 691,785 |
| Particles in final refinement | 110,958 |
| Refinement | |
| Resolution (Å) | 3.8 |
| No. atoms | 22117 |
| Protein | 21997 |
| RNA | 120 |
| Map sharpening B-factor | −153 |
| R.m.s deviations | |
| Bond lengths (Å) | 0.006 |
| Bond angles (°) | 0.926 |
| Ramachandran plot | |
| Favored (%) | 94.2 |
| Allowed (%) | 5.7 |
| Rotamers ouliers (%) | 0.65 |
| MapCC Global | 0.79 |