Fig. 4. (A) IFN-γ secretion from NK cells was suppressed by Fc-ARM. IGROV-1 cells coated with the anti-EGFR mAb (100 nM) and Fc-ARM 1 (10 nM) were co-incubated with NK cells (NK : IGROV-1 ratio = 1 : 1). Excess folate (10 μM) was added to suppress the complex formation on the cell surface. The amount of IFN-γ secreted from NK cells was quantitated by ELISA. (B) CLSM images of the recognition of anti-EGFR mAb-coated IGROV-1 cells by NK cells in the presence or absence of Fc-ARM 2. NK cells were transfected with ZsGreen. SeTau-647-labeled anti-EGFR mAb was used. The scale bars are 20 μm.
