Fig. 5. Flow cytometry analysis of HCC cell lines after 72 hours of treatment with quinoides 1–5. (A) Huh7, (B) Hep3B, (C) Mahlavu, and (D) SNU-475. Cancer stem cells were defined by their positivity for CSC markers, CD133 together with EpCAM, or CD90. Each treatment was compared to its corresponding DMSO control to define the changes in percentage of double positive population. DAPT is used as positive control for CSC inhibition. The bar graphs indicate the fold change in positivity for markers between DMSO control and experimental groups.