Fig. 3. Optimization of the concentration of L15 and co-solvent proportion in a plate format. Reaction conditions: A: 1a (1 nmol), 2b–j (500 mM), Cu(OAc)2 (25 mM), ligand L15 (50–200 mM), sodium ascorbate (50 mM), K3PO4 (500 mM), DMSO/water (1/3–3/1 16 μl), 40 °C, 3 h. The color and diameter of circles correlate with the yield of 3b–j determined by UPLC-TOF analysis. B: 1a (1 nmol), 2b–j (500 mM), Cu(OAc)2 (25 mM), ligand L15 (50–200 mM), sodium ascorbate (50 mM), K3PO4 (500 mM), DMSO/water (1/3–3/1 16 μl), 40 °C, 3 h. The color and diameter of circles correlate with the yield of 5a determined by UPLC-TOF analysis.
