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. 2018 May 11;17(8):1627–1636. doi: 10.1074/mcp.RA118.000767

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© 2018 Karim Malet et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

Author's Choice—Final version free via Creative Commons CC-BY license.

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Fig. 2. — Proteasome-independent and post-translational down-regulation of host proteins induced by LLO. A, Immunoblot analysis of HeLa cells pre-treated or not with proteasome inhibitors, and then incubated with 3 nm LLO for 10 or 30 min. Antibodies against the following targets were used to monitor changes in protein levels : CSTB (Cystatin-B), PCBP1 (Poly(rC)-binding protein 1), PHPT1 (14 kDa phospho-histidine phosphatase), PPIA (Peptidyl-prolyl cis-trans isomerase A), TXN (Thioredoxin), UBE2K (Ubiquitin-conjugating enzyme E2 K), UBE2N (Ubiquitin-conjugating enzyme E2 N) and UBC9 (SUMO-conjugating enzyme UBC9). B, Immunoblot analysis of HeLa cells pre-treated or not with cycloheximide (CHX) for 8 h, and then incubated with 3 nm LLO for 10 min. Actin is shown as a loading control.