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Host ubiquitylation alterations in response to Listeria infection. A, Immunoblot analysis of HeLa cells pre-treated or not with proteasome inhibitors, and then incubated with 3 nm LLO for 10 or 30 min. B, Immunoblot analysis of HeLa cells infected for 5 h with wild-type or Δhly Listeria monocytogenes. Antibodies against K48- and K63-linked polyubiquitin chains were used to monitor changes in host protein ubiquitination. Actin is shown as a loading control.
